Journal: Scientific reports
Article Title: Diesel exhaust particles induce human umbilical vein endothelial cells apoptosis by accumulation of autophagosomes and caspase-8 activation.
doi: 10.1038/s41598-022-21044-3
Figure Lengend Snippet: Figure 4. DEP impair autophagic flux in the degradation stage. (a) HUVECs infected with RFP-LC3B-GFP- expressing adenovirus were exposed to DEP (70 µg/ml) for 18 h and autolysosome formation was examined under fluorescence microscopy. (b) The number of yellow signals per cell was counted from 10 randomly selected cells. Statistical analysis was performed using two-tailed Student’s t test. **P < 0.02 versus control. (c) HUVECs were pre-incubated with Bafilomysin A1 (Baf A1, 100 nM) for 3 h and exposed to DEP (70 μg/ ml) for an additional 12 h. LC3B level was measured by immunoblotting. (d) Quantification of LC3BII levels normalized to β-actin. Results are presented as means ± SD (n = 3). Statistical analysis was performed using one-way ANOVA. **P < 0.02 versus no treatment. (e) HUVECs were exposed to DEP (70 μg/ml) for 24 h and protein levels of STX17, VAMP8, SNAP29, YKT6, STX7, and β-actin were measured by immunoblotting. (f) Quantification of STX17, VAMP8, SNAP29, YKT6, and STX7 levels normalized to β-actin. Results are presented as means ± SD (n = 3). Statistical analysis was performed using two-tailed Student’s t test. **P < 0.02 versus control.
Article Snippet: The STX7 antibody was purchased from Bethyl Laboratories Inc. (Montgomery, TX, USA).
Techniques: Infection, Expressing, Fluorescence, Microscopy, Two Tailed Test, Control, Incubation, Western Blot
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